Recombinant human SSX1 protein

Protein SSX1 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. SSX proteins are localized to the nucleus and expressed in testis and several types of cancers and, therefore, they are classified as C/T (cancer/testis) antigens. These proteins may function as transcriptional repressors. SSX1 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Recombinant human SSX1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Protein SSX1, CT5.1, SSRC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.3 kDa (211aa)

Additionnal Information

SSX1, Protein SSX1, CT5.1, SSRC, ATGP1985-10 µg, ATGP1985-20 µg, ATGP1985-50 µg, ATGP1985-100 µg, ATGP1985-250 µg, ATGP1985-500 µg, ATGP1985-1 mg, ATGP1985-10, ATGP1985-20, ATGP1985-50, ATGP1985-100, ATGP1985-250, ATGP1985-500, ATGP1985-1

References & Citations

Crew A J., et al. (1995) EMBO J. 14:2333-2340.; ; Nilsson G., et al. (1999) Cancer Res. 59:3180-3184.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005626

Uniprot Accession Number

Q16384

Species

Human

AA Sequence

MNGDDTF AKRPRDDAKA SEKRSKAFDD IATYFSKKEW KKMKYSEKIS YVYMKRNYKA MTKLGFKVTL PPFMCNKQAT DFQGNDFDND HNRRIQVEHP QMTFGRLHRI IPKIMPKKPA EDENDSKGVS EASGPQNDGK QLHPPGKANI SEKINKRSGP KRGKHAWTHR LRERKQLVIY EEISDPEEDD E