Recombinant human AIDA protein

AIDA acts as a ventralizing factor during embryogenesis. This protein inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling. Recombinant human AIDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Axin interactor dorsalization-associated, C1orf80, RP11-378J18.7

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.4 kDa (329aa) confirmed by MALDI-TOF

Additionnal Information

AIDA, Axin interactor dorsalization-associated, Axin interactor, dorsalization-associated, C1orf80, RP11-378J18.7, ATGP1977-10 µg, ATGP1977-20 µg, ATGP1977-50 µg, ATGP1977-100 µg, ATGP1977-250 µg, ATGP1977-500 µg, ATGP1977-1 mg, ATGP1977-10, ATGP1977-20, ATGP1977-50, ATGP1977-100, ATGP1977-250, ATGP1977-500, ATGP1977-1

References & Citations

Rui, Y., et al. (2007) Dev. Cell 13 (2), 268-282

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.2M NaCl,1mM DTT

Scientific Category

Development

NCBI Accession Number

NP_073742

Uniprot Accession Number

Q96BJ3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSEVTRS LLQRWGASFR RGADFDSWGQ LVEAIDEYQI LARHLQKEAQ AQHNNSEFTE EQKKTIGKIA TCLELRSAAL QSTQSQEEFK LEDLKKLEPI LKNILTYNKE FPFDVQPVPL RRILAPGEEE NLEFEEDEEE GGAGAGSPDS FPARVPGTLL PRLPSEPGMT LLTIRIEKIG LKDAGQCIDP YITVSVKDLN GIDLTPVQDT PVASRKEDTY VHFNVDIELQ KHVEKLTKGA AIFFEFKHYK PKKRFTSTKC FAFMEMDEIK PGPIVIELYK KPTDFKRKKL QLLTKKPLYL HLHQTLHKE