Recombinant human CD30/TNFRSF8 protein

TNFRSF8 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leadsto the activation of NF-kappaB. It is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Recombinant human TNFRSF8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, D1S166E

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

40.8 kDa (384aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30, D1S166E, KI-1, ATGP1976-10 µg, ATGP1976-20 µg, ATGP1976-50 µg, ATGP1976-100 µg, ATGP1976-250 µg, ATGP1976-500 µg, ATGP1976-1 mg, ATGP1976-10, ATGP1976-20, ATGP1976-50, ATGP1976-100, ATGP1976-250, ATGP1976-500, ATGP1976-1

References & Citations

Burgess, S.C., et al. (2004) Proc. Natl. Acad. Sci. u.S.A. 101 (38), 13879-13884; ; Berro, A.I., et al. (2004) J. Immunol. 173 (3), 2174-2183;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_001234.1

Uniprot Accession Number

P28908

Species

Human

AA Sequence

FPQDRPF EDTCHGNPSH YYDKAVRRCC YRCPMGLFPT QQCPQRPTDC RKQCEPDYYL DEADRCTACV TCSRDDLVEK TPCAWNSSRV CECRPGMFCS TSAVNSCARC FFHSVCPAGM IVKFPGTAQK NTVCEPASPG VSPACASPEN CKEPSSGTIP QAKPTPVSPA TSSASTMPVR GGTRLAQEAA SKLTRAPDSP SSVGRPSSDP GLSPTQPCPE GSGDCRKQCE PDYYLDEAGR CTACVSCSRD DLVEKTPCAW NSSRTCECRP GMICATSATN SCARCVPYPI CAAETVTKPQ DMAEKDTTFE APPLGTQPDC NPTPENGEAP ASTSPTQSLL VDSQASKTLP IPTSAPVALS STGK