Recombinant human ADI1 protein

ADI1 is an enzyme that belongs to the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Also, it down-regulates cell migration mediated by MMP14. Recombinant human ADI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.6 kDa (216aa) confirmed by MALDI-TOF

Additionnal Information

ADI1, Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL, ATGP1975-10 µg, ATGP1975-20 µg, ATGP1975-50 µg, ATGP1975-100 µg, ATGP1975-250 µg, ATGP1975-500 µg, ATGP1975-1 mg, ATGP1975-10, ATGP1975-20, ATGP1975-50, ATGP1975-100, ATGP1975-250, ATGP1975-500, ATGP1975-1

References & Citations

Gotoh, I., et al. (2007) Genes Cells 12 (1), 105-117

Other References

Bae DH, et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. (Biochim Biophys Acta Mol Basis Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/32480040/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_060739

Uniprot Accession Number

Q9BV57

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA