Recombinant human PHOSPHO1 protein

Phosphoethanolamine/phosphocholine phosphatase, also known as PHOSPHO1, has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) . This protein is involved in the generation of inorganic phosphate for bone mineralization. Recombinant human PHOSPHO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphoethanolamine/phosphocholine phosphatase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

32.2 kDa (291aa) confirmed by MALDI-TOF

Additionnal Information

PHOSPHO1, Phosphoethanolamine/phosphocholine phosphatase, ATGP1974-10 µg, ATGP1974-20 µg, ATGP1974-50 µg, ATGP1974-100 µg, ATGP1974-250 µg, ATGP1974-500 µg, ATGP1974-1 mg, ATGP1974-10, ATGP1974-20, ATGP1974-50, ATGP1974-100, ATGP1974-250, ATGP1974-500, ATGP1974-1

References & Citations

Roberts S.J., et al. (2004) Biochem. J. 382:59-65; ; Houston B R., et al. (2004) Bone. 34:629-637;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol

Scientific Category

Stem Cell

NCBI Accession Number

NP_848595

Uniprot Accession Number

Q8TCT1

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C