Recombinant human RPL12 protein

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL12 is a ribosomal protein that is a component of the 60S subunit. This protein belongs to the L11P family of ribosomal proteins. It is located in the cytoplasm. RPL12 binds directly to the 26S rRNA. Recombinant human RPL12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribosomal protein L12, L12

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.2 kDa (188aa) confirmed by MALDI-TOF

Additionnal Information

RPL12, Ribosomal protein L12, L12, ATGP1973-10 µg, ATGP1973-20 µg, ATGP1973-50 µg, ATGP1973-100 µg, ATGP1973-250 µg, ATGP1973-500 µg, ATGP1973-1 mg, ATGP1973-10, ATGP1973-20, ATGP1973-50, ATGP1973-100, ATGP1973-250, ATGP1973-500, ATGP1973-1

References & Citations

Rush J., et al. (2005) Nat. Biotechnol. 23:94-101; ; Daub H., et al. (2008) Mol. Cell. 31:438-448;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_000967

Uniprot Accession Number

P30050

Species

Human

AA Sequence

MPPKFDP NEIKVVYLRC TGGEVGATSA LAPKIGPLGL SPKKVGDDIA KATGDWKGLR ITVKLTIQNR QAQIEVVPSA SALIIKALKE PPRDRKKQKN IKHSGNITFD EIVNIARQMR HRSLARELSG TIKEILGTAQ SVGCNVDGRH PHDIIDDINS GAVECPAS