Recombinant human DAN protein

Neuroblastoma suppressor of tumorigenicity 1, also known as NBL1, is a member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. NBL1 is a candidate as a tumor suppressor of neuroblastoma and may play a role in preventing cells from entering the final stage (G1/S) of the transformation process. It is produced in small neurons of the dorsal root ganglion and its expression is activated by MATH-1. Recombinant human NBL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neuroblastoma suppressor of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20 kDa (187aa) confirmed by MALDI-TOF

Additionnal Information

DAN, Neuroblastoma suppressor of tumorigenicity 1, D1S1733E, DAN, DAND1, NB, NO3, ATGP1967-10 µg, ATGP1967-20 µg, ATGP1967-50 µg, ATGP1967-100 µg, ATGP1967-250 µg, ATGP1967-500 µg, ATGP1967-1 mg, ATGP1967-10, ATGP1967-20, ATGP1967-50, ATGP1967-100, ATGP1967-250, ATGP1967-500, ATGP1967-1

References & Citations

Kim A S., et al. (2003) Brain Res Dev. 145:159-162; ; Gerlach Bank L M., et al. (2004) Dev Dyn. 229: 219-230.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_005371

Uniprot Accession Number

P41271

Species

Human

AA Sequence

APPPINK LALFPDKSAW CEAKNITQIV GHSGCEAKSI QNRACLGQCF SYSVPNTFPQ STESLVHCDS CMPAQSMWEI VTLECPGHEE VPRVDKLVEK ILHCSCQACG KEPSHEGLSV YVQGEDGPGS QPGTHPHPHP HPHPGGQTPE PEDPPGAPHT EEEGAED