Recombinant human RAP2B protein

Ras-related protein Rap-2b, also known as RAP2B, belongs to a family of RAS-related genes. unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. RAP2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway. Recombinant human RAP2B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Rap-2b, RAP2B, Member of RAS oncogene family, Small GTP binding protein, Ras family small GTP binding protein RAP2B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.9 kDa (206aa) confirmed by MALDI-TOF

Additionnal Information

Small GTP binding protein, Ras-related protein Rap-2b, Ras-related protein RAP2B, Ras related protein RAP2B, Ras related protein Rap 2b, Ras family small GTP binding protein RAP2B, RAP2B, P61225, Member of RAS oncogene family, ATGP1966-10 µg, ATGP1966-20 µg, ATGP1966-50 µg, ATGP1966-100 µg, ATGP1966-250 µg, ATGP1966-500 µg, ATGP1966-1 mg, ATGP1966-010, ATGP1966-020, ATGP1966-050, ATGP1966-100, ATGP1966-250, ATGP1966-500, ATGP1966-01M

References & Citations

Culine S., et al. (1989) Int J Cancer. 44:990-994; ; Beranger F., et al. (1991) Oncogene. 6: 1835-1842.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002877

Uniprot Accession Number

P61225

Species

Human

AA Sequence

MREYKVV VLGSGGVGKS ALTVQFVTGS FIEKYDPTIE DFYRKEIEVD SSPSVLEILD TAGTEQFASM RDLYIKNGQG FILVYSLVNQ QSFQDIKPMR DQIIRVKRYE RVPMILVGNK VDLEGEREVS YGEGKALAEE WSCPFMETSA KNKASVDELF AEIVRQMNYA AQPNGDEGCC SACVIL