Recombinant human NUDT14/UGPP protein

Uridine diphosphate glucose pyrophosphatase, also known as NuDT14, is a 222 amino acid cytoplasmic protein that contains one nudix hydrolase domain and belongs to the nudix hydrolase family. NuDT14 hydrolyzes ADP-ribose into ribose 5-phosphate and AMP, and uDP-glucose to glucose 1-phosphate and uMP. Existing as a homodimer, NuDT14 binds magnesium as a cofactor and is encoded by a gene located on human chromosome 14. Recombinant human NuDT14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nudix hydrolase 14, Uridine diphosphate glucose pyrophosphatase NUDT14, UDPG pyrophosphatase, UGPPase, Nucleoside diphosphate-linked moiety X motif 14, Nudix motif 14

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.5 kDa (245aa) confirmed by MALDI-TOF

Additionnal Information

NUDT14, uridine diphosphate glucose pyrophosphatase, uGPP, uGPPase, ATGP1965-10 µg, ATGP1965-20 µg, ATGP1965-50 µg, ATGP1965-100 µg, ATGP1965-250 µg, ATGP1965-500 µg, ATGP1965-1 mg, ATGP1965-10, ATGP1965-20, ATGP1965-50, ATGP1965-100, ATGP1965-250, ATGP1965-500, ATGP1965-1

References & Citations

Yagi T., et al. (2003) Biochem J. 370:409-415.; ; Heyen C A., et al. (2009) Biochem Biophys Res Commun. 390: 1414-1418.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_803877.2

Uniprot Accession Number

O95848

Species

Human

AA Sequence

MERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ