Recombinant human CD34 protein

Hematopoietic progenitor cell antigen CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is likely an adhesion molecule with a role in early hematopoiesis by mediating attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Recombinant human CD34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hematopoietic progenitor cell antigen CD34, CD34 molecule

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.7 kDa (280aa) confirmed by MALDI-TOF

Additionnal Information

CD34, Hematopoietic progenitor cell antigen CD34, CD34 molecule, ATGP1964-10 µg, ATGP1964-20 µg, ATGP1964-50 µg, ATGP1964-100 µg, ATGP1964-250 µg, ATGP1964-500 µg, ATGP1964-1 mg, ATGP1964-10, ATGP1964-20, ATGP1964-50, ATGP1964-100, ATGP1964-250, ATGP1964-500, ATGP1964-1

References & Citations

Satterthwaite A B., et al. (1992) Genomics. 12: 788-794; ; Krause D S., et al. (1994) Blood. 84: 691-701.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Stem Cell

NCBI Accession Number

NP_001020280

Uniprot Accession Number

P28906

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSLDNNGTAT PELPTQGTFS NVSTNVSYQE TTTPSTLGST SLHPVSQHGN EATTNITETT VKFTSTSVIT SVYGNTNSSV QSQTSVISTV FTTPANVSTP ETTLKPSLSP GNVSDLSTTS TSLATSPTKP YTSSSPILSD IKAEIKCSGI REVKLTQGIC LEQNKTSSCA EFKKDRGEGL ARVLCGEEQA DADAGAQVCS LLLAQSEVRP QCLLLVLANR TEISSKLQLM KKHQSDLKKL GILDFTEQDV ASHQSYSQKT