Recombinant human CD34 protein
Hematopoietic progenitor cell antigen CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is likely an adhesion molecule with a role in early hematopoiesis by mediating attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Recombinant human CD34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Hematopoietic progenitor cell antigen CD34, CD34 molecule
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.7 kDa (280aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Satterthwaite A B., et al. (1992) Genomics. 12: 788-794; ; Krause D S., et al. (1994) Blood. 84: 691-701.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Stem Cell
NCBI Accession Number
NP_001020280
Uniprot Accession Number
P28906
Species
Human
AA Sequence
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