Recombinant human RAB10 protein

RAB10 is a member of the Ras superfamily of small GTP-binding proteins. This protein is a key regulator of endocytic recycling, especially in the basolateral recycling pathways of polarized epithelial cells. It is required for the recruitment of CNT-1 to endosomal membranes in the intestinal epithelium. Recombinant human RAB10 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Ras-related GTP-binding protein RAB10, RAB10, RAB10 member RAS oncogene family, Ras-related protein Rab-10

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.7 kDa (220aa)

Additionnal Information

RAB10, ATGP1963-10 µg, ATGP1963-20 µg, ATGP1963-50 µg, ATGP1963-100 µg, ATGP1963-250 µg, ATGP1963-500 µg, ATGP1963-1 mg, ATGP1963-10, ATGP1963-20, ATGP1963-50, ATGP1963-100, ATGP1963-250, ATGP1963-500, ATGP1963-1

References & Citations

Chen Y. et al. (2012) J Cell Biol. 198:545-560.; ; Sano H. et al. (2011) J Biol Chem. 286:16541-16545.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057215.3

Uniprot Accession Number

P61026

Species

Human

AA Sequence

MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC