Recombinant human Cytokeratin 20/KRT20 protein

KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. KRT20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. Recombinant human KRT20 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

50.9 kDa (447aa)

Additionnal Information

KRT20, Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20; CK-20; CK20; K20; KRT21, ATGP1960-10 µg, ATGP1960-20 µg, ATGP1960-50 µg, ATGP1960-100 µg, ATGP1960-250 µg, ATGP1960-500 µg, ATGP1960-1 mg, ATGP1960-10, ATGP1960-20, ATGP1960-50, ATGP1960-100, ATGP1960-250, ATGP1960-500, ATGP1960-1

References & Citations

Schweizer J, Bowden PE, et al. (2006) . J Cell Biol.174 (2) :169-74.

Other References

Hoshino A, et al. Identification of autoantibodies using human proteome microarrays in patients with IPEX syndrome. (Clin Immunol. 2019) {https://pubmed.ncbi.nlm.nih.gov/30951839/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_061883

Uniprot Accession Number

P35900

Species

Human

AA Sequence

MDFSRRS FHRSLSSSLQ APVVSTVGMQ RLGTTPSVYG GAGGRGIRIS NSRHTVNYGS DLTGGGDLFV GNEKMAMQNL NDRLASYLEK VRTLEQSNSK LEVQIKQWYE TNAPRAGRDY SAYYRQIEEL RSQIKDAQLQ NARCVLQIDN AKLAAEDFRL KYETERGIRL TVEADLQGLN KVFDDLTLHK TDLEIQIEEL NKDLALLKKE HQEEVDGLHK HLGNTVNVEV DAAPGLNLGV IMNEMRQKYE VMAQKNLQEA KEQFERQTAV LQQQVTVNTE ELKGTEVQLT ELRRTSQSLE IELQSHLSMK ESLEHTLEET KARYSSQLAN LQSLLSSLEA QLMQIRSNME RQNNEYHILL DIKTRLEQEI ATYRRLLEGE DVKTTEYQLS TLEERDIKKT RKIKTVVQEV VDGKVVSSEV KEVEENI