Recombinant human SFRS1 protein

Serine/arginine-rich splicing factor 1, also known as SFRS1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Recombinant human SFRS1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Serine/arginine-rich splicing factor 1, ASF, MGC5228, SF2, SF2p33, SRp30a

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.9 kDa (268aa)

Additionnal Information

SFRS1, Serine/arginine-rich splicing factor 1, ASF, MGC5228, SF2, SF2p33, SRp30a, ATGP1949-10 µg, ATGP1949-20 µg, ATGP1949-50 µg, ATGP1949-100 µg, ATGP1949-250 µg, ATGP1949-500 µg, ATGP1949-1 mg, ATGP1949-10, ATGP1949-20, ATGP1949-50, ATGP1949-100, ATGP1949-250, ATGP1949-500, ATGP1949-1

References & Citations

Zuo P., et al. (1993) EMBO J. 12:4727-4737; ; Kohtz J.D., et al. (1994) Nature. 368:119-124;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_008855

Uniprot Accession Number

Q07955

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRT