Recombinant human Oct-3/4 protein

POu domain, class 5, transcription factor 1, also known as OCT4, binds to the octamer motif (5'-ATTTGCAT-3') . This protein forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, uTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency. Recombinant human OCT4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

POU domain, class 5, transcription factor 1 isoform 3, Oct-3, Oct-4, OCT3, OCT4, OTF-3, OTF3, OTF4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.2 kDa (285aa)

Additionnal Information

38263, POU domain, class 5, transcription factor 1 isoform 3, Oct-3, Oct-4, OCT3, OCT4, OTF-3, OTF3, OTF4, ATGP1948-10 µg, ATGP1948-20 µg, ATGP1948-50 µg, ATGP1948-100 µg, ATGP1948-250 µg, ATGP1948-500 µg, ATGP1948-1 mg, ATGP1948-10, ATGP1948-20, ATGP1948-50, ATGP1948-100, ATGP1948-250, ATGP1948-500, ATGP1948-1

References & Citations

Takahashi K., et al. (2007) Cell. 131:861-872; ; Lee J., et al. (2008) Int. J. Biochem. Cell Biol. 40:1043-1054

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Development

NCBI Accession Number

NP_001272916

Uniprot Accession Number

Q01860

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MHFYRLFLGA TRRFLNPEWK GEIDNWCVYV LTSLLPFKIQ SQDIKALQKE LEQFAKLLKQ KRITLGYTQA DVGLTLGVLF GKVFSQTTIC RFEALQLSFK NMCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN