Recombinant human LITAF protein

Lipopolysaccharide-induced TNF-alpha factor, also known as LITAF, is a small integral membrane protein of lysosome/late endosome. LITAF mediates the expression of inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced processes. LITAF binds to STAT6B, a member of the STAT6 family forming a complex on the TNF-alpha promoter that modulates TNF activity. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. Recombinant human LITAF protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Lipopolysaccharide-induced TNF-alpha factor, PIG7, SIMPLE

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

19.2 kDa (181aa)

Additionnal Information

LITAF, Lipopolysaccharide-induced TNF-alpha factor, PIG7, SIMPLE, ATGP1946-10 µg, ATGP1946-20 µg, ATGP1946-50 µg, ATGP1946-100 µg, ATGP1946-250 µg, ATGP1946-500 µg, ATGP1946-1 mg, ATGP1946-10, ATGP1946-20, ATGP1946-50, ATGP1946-100, ATGP1946-250, ATGP1946-500, ATGP1946-1

References & Citations

Atucchi A., et al. (2006) Inflamm Bowel Dis. 13 (1) :120-121.; ; Tang X., et al. (2005) Proc Natl Acad Sci uSA. 102 (14) :5132-5137.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004853

Uniprot Accession Number

Q99732

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSVPGPYQAA TGPSSAPSAP PSYEETVAVN SYYPTPPAPM PGPTTGLVTG PDGKGMNPPS YYTQPAPIPN NNPITVQTVY VQHPITFLDR PIQMCCPSCN KMIVSQLSYN AGALTWLSCG SLCLLGCIAG CCFIPFCVDA LQDVDHYCPN CRALLGTYKR L