Recombinant human DEDD protein

Death effector domain-containing protein, also known as DEDD, is a cytoplasmic protein. It translocates to the nucleus during CD95-mediated apoptosis, where it localizes to nucleoli-like structures, activates caspase-6 and specifically inhibits RNA polymerase I-dependent transcription. The cell death activity of DEDD relates to its nuclear localization. DEDD is widely expressed in a variety of tissues, with highest levels in the testis. Overexpression of this gene was shown to induce weak apoptosis. Recombinant human DEDD protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Death effector domain-containing protein, CASP8IP1, DEDD1, DEFT, FLDED1, KE05

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

38.9 kDa (338aa)

Additionnal Information

DEDD, Death effector domain-containing protein, CASP8IP1, DEDD1, DEFT, FLDED1, KE05, ATGP1945-10 µg, ATGP1945-20 µg, ATGP1945-50 µg, ATGP1945-100 µg, ATGP1945-250 µg, ATGP1945-500 µg, ATGP1945-1 mg, ATGP1945-10, ATGP1945-20, ATGP1945-50, ATGP1945-100, ATGP1945-250, ATGP1945-500, ATGP1945-1

References & Citations

Stegh A H., et al. (1998) EMBO J. 17:5974-5986.; ; Schickling O., et al. (2001) Cell Death Differ. 8:1157-1168.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_127491

Uniprot Accession Number

O75618

Species

Human

AA Sequence

MAGLKRRASQ VWPEEHGEQE HGLYSLHRMF DIVGTHLTHR DVRVLSFLFV DVIDDHERGL IRNGRDFLLA LERQGRCDES NFRQVLQLLR IITRHDLLPY VTLKRRRAVC PDLVDKYLEE TSIRYVTPRA LSDPEPRPPQ PSKTVPPHYP VVCCPTSGPQ MCSKRPARGR ATLGSQRKRR KSVTPDPKEK QTCDIRLRVR AEYCQHETAL QGNVFSNKQD PLERQFERFN QANTILKSRD LGSIICDIKF SELTYLDAFW RDYINGSLLE ALKGVFITDS LKQAVGHEAI KLLVNVDEED YELGRQKLLR NLMLQALP