Recombinant human Hexosaminidase A/HEXA protein
HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I) . Recombinant human HEXA protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Beta-hexosaminidase subunit alpha, TSD, hexosaminidase A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
52.9 kDa (464aa)
Additionnal Information
References & Citations
Nakano T., et al. (1988) J. Neurochem. 51:984-987
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_000511.1
Uniprot Accession Number
P06865
Species
Human
AA Sequence
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