Recombinant human Cytokeratin 19 protein

KRT19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Recombinant human KRT19 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, CK19, K19, K1CS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

46.5 kDa (423aa)

Additionnal Information

Cytokeratin19, Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, CK19, K19, K1CS, ATGP1939-10 µg, ATGP1939-20 µg, ATGP1939-50 µg, ATGP1939-100 µg, ATGP1939-250 µg, ATGP1939-500 µg, ATGP1939-1 mg, ATGP1939-10, ATGP1939-20, ATGP1939-50, ATGP1939-100, ATGP1939-250, ATGP1939-500, ATGP1939-1

References & Citations

Vilardell, F., et al. (2012) Virchows Arch. 460 (6), 569-575; ; Leelawat, K., et al. (2012) World J. Gastroenterol. 18 (2), 175-181

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M uREA, 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_002267

Uniprot Accession Number

P08727

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTSYSYR QSSATSSFGG LGGGSVRFGP GVAFRAPSIH GGSGGRGVSV SSARFVSSSS SGAYGGGYGG VLTASDGLLA GNEKLTMQNL NDRLASYLDK VRALEAANGE LEVKIRDWYQ KQGPGPSRDY SHYYTTIQDL RDKILGATIE NSRIVLQIDN ARLAADDFRT KFETEQALRM SVEADINGLR RVLDELTLAR TDLEMQIEGL KEELAYLKKN HEEEISTLRG QVGGQVSVEV DSAPGTDLAK ILSDMRSQYE VMAEQNRKDA EAWFTSRTEE LNREVAGHTE QLQMSRSEVT DLRRTLQGLE IELQSQLSMK AALEDTLAET EARFGAQLAH IQALISGIEA QLGDVRADSE RQNQEYQRLM DIKSRLEQEI ATYRSLLEGQ EDHYNNLSAS KVL