Recombinant human CD32a/FCGR2A protein

Low affinity immunoglobulin gamma Fc region receptor II-a, also known as FCGR2A, is a member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. This protein is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Recombinant human FCGR2A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc gamma receptor Iia, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32A, FCG2, FCGR2A1, IGFR2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.0 kDa (208aa) confirmed by MALDI-TOF

Additionnal Information

Fc g RIIA, CD32a, Low affinity immunoglobulin gamma Fc region receptor II-a, CD32, CD32A, CDw32, FcGR, IGFR2, MGC23887, MGC30032, ATGP1936-10 µg, ATGP1936-20 µg, ATGP1936-50 µg, ATGP1936-100 µg, ATGP1936-250 µg, ATGP1936-500 µg, ATGP1936-1 mg, ATGP1936-10, ATGP1936-20, ATGP1936-50, ATGP1936-100, ATGP1936-250, ATGP1936-500, ATGP1936-1

References & Citations

Lu J., et al. (2008) Nature. 456:989-992; ; Rikova K., et al. (2007) Cell. 131:1190-1203

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 50% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

AAH19931.1

Uniprot Accession Number

P12318

Species

Human

AA Sequence

QAAPP KAVLKLEPPW INVLQEDSVT LTCQGARSPE SDSIQWFHNG NLIPTHTQPS YRFKANNNDS GEYTCQTGQT SLSDPVHLTV LSEWLVLQTP HLEFQEGETI MLRCHSWKDK PLVKVTFFQN GKSQKFSRLD PTFSIPQANH SHSGDYHCTG NIGYTLFSSK PVTITVQVPS MGSSSPMG