Recombinant human OBFC1 protein

CST complex subunit STN1, also known as OBFC1, is a component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. Recombinant human OBFC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CST complex subunit STN1, AAF-44, RPA-32, STN1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

44.5 kDa (391aa) confirmed by MALDI-TOF

Additionnal Information

OBFC1, CST complex subunit STN1, AAF-44, RPA-32, STN1, ATGP1935-10 µg, ATGP1935-20 µg, ATGP1935-50 µg, ATGP1935-100 µg, ATGP1935-250 µg, ATGP1935-500 µg, ATGP1935-1 mg, ATGP1935-10, ATGP1935-20, ATGP1935-50, ATGP1935-100, ATGP1935-250, ATGP1935-500, ATGP1935-1

References & Citations

Miyake Y., et al. (2009) Mol. Cell. 36:193-206; ; Wan M., et al. (2009) J. Biol. Chem. 284:26725-26731

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_079204.1

Uniprot Accession Number

Q9H668

Species

Human

AA Sequence

MQPGSSR CEEETPSLLW GLDPVFLAFA KLYIRDILDM KESRQVPGVF LYNGHPIKQV DVLGTVIGVR ERDAFYSYGV DDSTGVINCI CWKKLNTESV SAAPSAAREL SLTSQLKKLQ ETIEQKTKIE IGDTIRVRGS IRTYREEREI HATAYYKVDD PVWNIQIARM LELPTIYRKV YDQPFHSSAL EKEEALSNPG ALDLPSLTSL LSEKAKEFLM ENRVQSFYQQ ELEMVESLLS LANQPVIHSA CSDQVNFKKD TTSKAIHSIF KNAIQLLQEK GLVFQKDDGF DNLYYVTRED KDLHRKIHRI IQQDCQKPNH MEKGCHFLHI LACARLSIRP GLSEAVLQQV LELLEDQSDI VSTMEHYYTA F