Recombinant human MafK protein

MAFK belongs to the bZIP family and Maf subfamily. Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. Small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Recombinant human MAFK protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Transcription factor MafK, NFE2u, P18

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

19.7 kDa (176aa)

Additionnal Information

MafK, Transcription factor MafK, NFE2u, P18, ATGP1931-10 µg, ATGP1931-20 µg, ATGP1931-50 µg, ATGP1931-100 µg, ATGP1931-250 µg, ATGP1931-500 µg, ATGP1931-1 mg, ATGP1931-10, ATGP1931-20, ATGP1931-50, ATGP1931-100, ATGP1931-250, ATGP1931-500, ATGP1931-1

References & Citations

Iwata T, et al. (1998) Cytogenet Cell Genet 82 (1-2) : 88-90.; ; Du MJ, et al. (2008) Int J Biochem Cell Biol. 40 (8) :1481-93.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002351

Uniprot Accession Number

O60675

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS