Recombinant human Bub3 protein
Mitotic checkpoint protein BuB3, also known as BuB3, is a conserved component of the mitotic spindle assembly complex (MCC) . The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BuB3 protein. BuB3 is essential for the kinetochore localization of BuB1 and BuBR1. BuB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. In addition, BuB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. Recombinant human BuB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
Mitotic checkpoint protein BuB3, BuB3L, hBuB3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
39.5 kDa (351aa)
Additionnal Information
References & Citations
Martinez Exposito M J., et al. (1999) Proc Natl Acad Sci uSA. 96:8493-8498.; ; Warren C D., et al. (2002) Mol Biol Cell. 13:3029-3041.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004716
Uniprot Accession Number
O43684
Species
Human
AA Sequence
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