Recombinant human MIS12 protein

Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization. Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Protein MIS12 homolog, hMis12, KNTC2AP, MTW1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.5 kDa (228aa)

Additionnal Information

MIS12, Protein MIS12 homolog, hMis12, KNTC2AP, MTW1, ATGP1928-10 µg, ATGP1928-20 µg, ATGP1928-50 µg, ATGP1928-100 µg, ATGP1928-250 µg, ATGP1928-500 µg, ATGP1928-1 mg, ATGP1928-10, ATGP1928-20, ATGP1928-50, ATGP1928-100, ATGP1928-250, ATGP1928-500, ATGP1928-1

References & Citations

Kline S L., et al. (2006) J Cell Biol. 173:9-17.; ; Goshima G., et al. (2003) J Cell Biol. 160:25-39.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_076944

Uniprot Accession Number

Q9H081

Species

Human

AA Sequence

MSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS