Recombinant human HBG2 protein

Hemoglobin subunit gamma-2, also known as HBG2, belongs to the fetal hemoglobin subunit, which consists of two alpha chains together with two gamma chains. Increased fetal hemoglobin production in adults can ameliorate the clinical severity of sickle cell disease and beta-thalassemia major. Recombinant human HBG2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hemoglobin subunit gamma-2, TNCY

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.5 kDa (170aa) confirmed by MALDI-TOF

Additionnal Information

HBG2, ATGP1927-10 µg, ATGP1927-20 µg, ATGP1927-50 µg, ATGP1927-100 µg, ATGP1927-250 µg, ATGP1927-500 µg, ATGP1927-1 mg, ATGP1927-10, ATGP1927-20, ATGP1927-50, ATGP1927-100, ATGP1927-250, ATGP1927-500, ATGP1927-1

References & Citations

Feng L., et al. (2004) Cell. 119:629-640.; ; Liebhaber S A., et al. (1981) Nature.290:26-29.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol,1mM DTT

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000175.1

Uniprot Accession Number

P69892

Species

Human

AA Sequence

MGHFTEE DKATITSLWG KVNVEDAGGE TLGRLLVVYP WTQRFFDSFG NLSSASAIMG NPKVKAHGKK VLTSLGDAIK HLDDLKGTFA QLSELHCDKL HVDPENFKLL GNVLVTVLAI HFGKEFTPEV QASWQKMVTG VASALSSRYH