Recombinant human CD83 protein
CD83 is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human CD83 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
CD83 antigen isoform b precursor, BL11, HB15
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
16 kDa (148aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhou LJ, Tedder TF, et al. (1995) . J Immunol. 154 (8) :3821-35.; ; Lechmann M, Berchtold S, et al. (2002) . Trends Immunol. 23 (6) :273-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001035370
Uniprot Accession Number
Q01151
Species
Human
AA Sequence
TPEVKVA CSEDVDLPCT APWDPQVPYT VSWVKLLEGG EERMETPQED HLRGQHYHQK GQNGSFDAPN ERPYSLKIRN TTSCNSGTYR CTLQDPDGQR NLSGKVILRV TGCPAQRKEE TFKKYRAE
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