Recombinant human PRND protein
PRND is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. This protein is expressed during embryogenesis, but is expressed minimally in the central nervous system. Its function is still unknown. Mutations in PRND may lead to neurological disorders. Recombinant human PRND protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Prion-like protein doppel, Prion protein 2, DOPPEL, DPL, PrPLP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
16.9 kDa (149aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Peoc'h K, Guerin C, et al. (2000) . Neurosci Lett. 286 (2) :144-8.; ; Serres C, Peoc'h K, et al. (2006) . Biol Reprod. 74 (5) :816-23.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Neuroscience
NCBI Accession Number
NP_036541
Uniprot Accession Number
Q9UKY0
Species
Human
AA Sequence
RGIKHRI KWNRKALPST AQITEAQVAE NRPGAFIKQG RKLDIDFGAE GNRYYEANYW QFPDGIHYNG CSEANVTKEA FVTGCINATQ AANQGEFQKP DNKLHQQVLW RLVQELCSLK HCEFWLERG
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