Recombinant human Guanine deaminase/GDA protein

GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Guanine deaminase isoform b, Guanase, Guanine aminase, Guanine aminohydrolase, GAH, p51-nedasin, CYPIN, Cytoplasmic PSD-95 interactor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

53 kDa (477aa)

Additionnal Information

GDA, Guanine deaminase, CYPIN, GuANASE, NEDASIN, ATGP1922-10 µg, ATGP1922-20 µg, ATGP1922-50 µg, ATGP1922-100 µg, ATGP1922-250 µg, ATGP1922-500 µg, ATGP1922-1 mg, ATGP1922-10, ATGP1922-20, ATGP1922-50, ATGP1922-100, ATGP1922-250, ATGP1922-500, ATGP1922-1

References & Citations

Gang Yuan, James C. Bin, et al. (1999) . J. Biol. Chem. 274 (12) :8175-80.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004284

Uniprot Accession Number

Q9Y2T3

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV