Recombinant human USP14 protein

USP14 is a member of the ubiquitin-specific processing (uBP) family of proteases that is a deubiquitinating enzyme (DuB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Recombinant human uSP14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase 14, TGT, ubiquitin thioesterase 14

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

58.5 kDa (517aa)

Additionnal Information

USP14, ubiquitin carboxyl-terminal hydrolase 14, TGT, ubiquitin thioesterase 14, ATGP1921-10 µg, ATGP1921-20 µg, ATGP1921-50 µg, ATGP1921-100 µg, ATGP1921-250 µg, ATGP1921-500 µg, ATGP1921-1 mg, ATGP1921-10, ATGP1921-20, ATGP1921-50, ATGP1921-100, ATGP1921-250, ATGP1921-500, ATGP1921-1

References & Citations

Shinji S, Naito Z, et al. (2009) . Oncol Rep. 15 (3) :539-43.; ; Mines MA, Goodwin JS, et al. (2009) . J Biol Chem. 284 (9) :5742-52.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005142

Uniprot Accession Number

P54578

Species

Human

AA Sequence

MPLYSVT VKWGKEKFEG VELNTDEPPM VFKAQLFALT GVQPARQKVM VKGGTLKDDD WGNIKIKNGM TLLMMGSADA LPEEPSAKTV FVEDMTEEQL ASAMELPCGL TNLGNTCYMN ATVQCIRSVP ELKDALKRYA GALRASGEMA SAQYITAALR DLFDSMDKTS SSIPPIILLQ FLHMAFPQFA EKGEQGQYLQ QDANECWIQM MRVLQQKLEA IEDDSVKETD SSSASAATPS KKKSLIDQFF GVEFETTMKC TESEEEEVTK GKENQLQLSC FINQEVKYLF TGLKLRLQEE ITKQSPTLQR NALYIKSSKI SRLPAYLTIQ MVRFFYKEKE SVNAKVLKDV KFPLMLDMYE LCTPELQEKM VSFRSKFKDL EDKKVNQQPN TSDKKSSPQK EVKYEPFSFA DDIGSNNCGY YDLQAVLTHQ GRSSSSGHYV SWVKRKQDEW IKFDDDKVSI VTPEDILRLS GGGDWHIAYV LLYGPRRVEI MEEESEQ