Recombinant human Lyn protein

Tyrosine-protein kinase Lyn, also known as LYN, is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. This protein is Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. It Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. Recombinant human LYN protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Tyrosine-protein kinase Lyn, JTK8, p53Lyn, p56Lyn

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

61.0 kDa (535aa)

Additionnal Information

Lyn, Tyrosine-protein kinase Lyn, JTK8, p53Lyn, p56Lyn, ATGP1918-10 µg, ATGP1918-20 µg, ATGP1918-50 µg, ATGP1918-100 µg, ATGP1918-250 µg, ATGP1918-500 µg, ATGP1918-1 mg, ATGP1918-10, ATGP1918-20, ATGP1918-50, ATGP1918-100, ATGP1918-250, ATGP1918-500, ATGP1918-1

References & Citations

Gaul B.S., et al. (2001) J. Biol. Chem. Res. 275:16174-16182; ; Rena G., et al. (2001) Mol. Pharmacol. 59:996-1011

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002341

Uniprot Accession Number

P07948

Species

Human

AA Sequence

MGCIKSK GKDSLSDDGV DLKTQPVRNT ERTIYVRDPT SNKQQRPVPE SQLLPGQRFQ TKDPEEQGDI VVALYPYDGI HPDDLSFKKG EKMKVLEEHG EWWKAKSLLT KKEGFIPSNY VAKLNTLETE EWFFKDITRK DAERQLLAPG NSAGAFLIRE SETLKGSFSL SVRDFDPVHG DVIKHYKIRS LDNGGYYISP RITFPCISDM IKHYQKQADG LCRRLEKACI SPKPQKPWDK DAWEIPRESI KLVKRLGAGQ FGEVWMGYYN NSTKVAVKTL KPGTMSVQAF LEEANLMKTL QHDKLVRLYA VVTREEPIYI ITEYMAKGSL LDFLKSDEGG KVLLPKLIDF SAQIAEGMAY IERKNYIHRD LRAANVLVSE SLMCKIADFG LARVIEDNEY TAREGAKFPI KWTAPEAINF GCFTIKSDVW SFGILLYEIV TYGKIPYPGR TNADVMTALS QGYRMPRVEN CPDELYDIMK MCWKEKAEER PTFDYLQSVL DDFYTATEGQ YQQQP