Recombinant human CNDP2/CPGL protein

Cytosolic non-specific dipeptidase, also known as CNDP2, is a cytosolic, non-specific dipeptidase that belongs to the peptidase M20A family of proteins. CNDP2 is a secreted peptidase homologous to M20 peptidases. It is expressed by all adult and fetal tissue, however, an isoform lacking exons 3 and 4 was expressed in all fetal tissue, but only in adult liver. Overexpression of CPGL-B in hepatocellular carcinoma cells leads to significant inhibition of HC cell viability, colony formation, cell invasiveness and tumor formation. Recombinant human CNDP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytosolic non-specific dipeptidase, CN2, CPGL, HsT2298, PEPA

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

55.3 kDa (498aa)

Additionnal Information

CNDP2, CPGL, Cytosolic non-specific dipeptidase, CN2, CPGL, HsT2298, PEPA, ATGP1915-10 µg, ATGP1915-20 µg, ATGP1915-50 µg, ATGP1915-100 µg, ATGP1915-250 µg, ATGP1915-500 µg, ATGP1915-1 mg, ATGP1915-10, ATGP1915-20, ATGP1915-50, ATGP1915-100, ATGP1915-250, ATGP1915-500, ATGP1915-1

References & Citations

Wanic K., et al. (2008) Diabetes. 57:2547-2551.; ; Otani H., et al. (2008) Neurosci Lett. 445:166-169.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_060705

Uniprot Accession Number

Q96KP4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAALTTL FKYIDENQDR YIKKLAKWVA IQSVSAWPEK RGEIRRMMEV AAADVKQLGG SVELVDIGKQ KLPDGSEIPL PPILLGRLGS DPQKKTVCIY GHLDVQPAAL EDGWDSEPFT LVERDGKLYG RGSTDDKGPV AGWINALEAY QKTGQEIPVN VRFCLEGMEE SGSEGLDELI FARKDTFFKD VDYVCISDNY WLGKKKPCIT YGLRGICYFF IEVECSNKDL HSGVYGGSVH EAMTDLILLM GSLVDKRGNI LIPGINEAVA AVTEEEHKLY DDIDFDIEEF AKDVGAQILL HSHKKDILMH RWRYPSLSLH GIEGAFSGSG AKTVIPRKVV GKFSIRLVPN MTPEVVGEQV TSYLTKKFAE LRSPNEFKVY MGHGGKPWVS DFSHPHYLAG RRAMKTVFGV EPDLTREGGS IPVTLTFQEA TGKNVMLLPV GSADDGAHSQ NEKLNRYNYI EGTKMLAAYL YEVSQLKD