Recombinant human O-fucosyltransferase 1/POFUT1 protein
GDP-fucose protein O-fucosyltransferase 1, also known as POuFuT1, is a member of the glycosyltransferase O-Fuc family. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFuT1 uses manganese to catalyze the attachment of fucose to a conserved serine or threonine residue on a protein accept. POFuT1 plays an important role in Notch signaling, as Notch ligands can serve as POFuT1 substrates. Recombinant human POFuT1 protein, fused to His-tag at N-terminus, was expressed in E. coli.
Product Specifications
Product Name Alternative
GDP-fucose protein O-fucosyltransferase 1, FuT12, O-Fuc-T, O-FucT-1, O-FuT
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Denatured
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
43.7 kDa (385aa)
Additionnal Information
References & Citations
Wang Y., et al. (2001) J Biol Chem. 276:40338-40345.; ; Luo Y., et al. (2005) J Biol Chem. 280:11289-11294.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2M urea, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_056167
Uniprot Accession Number
Q9H488
Species
Human
AA Sequence
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