Recombinant human PLUNC protein

BPIFA1, also known as PLuNC, belongs to the short subfamily of PLuNC family proteins and have homology only to the Nterminal domains of BPI. It is a secreted protein that is expressed in the secretory ducts and submucosal glands of tracheobronchial tissues. BPIFA1 binds to lipopolysaccharide (LPS) in nasal lavage fluid (NLF) which points to its role in the inflammatory response of the upper airways after exposure to irritants. Decreased levels of BPIFA1 occur in the NLF of smokers and people who have been exposed to reactive epoxy chemicals, indicating that long-term exposure to airway irritants impairs the production of BPIFA1 in the upper respiratory tract. Recombinant human BPIFA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

BPI fold-containing family A member 1, BPI fold-containing family A, member 1, bA49G10.5, LPLuNC3, LuNX, NASG, PLuNC, SPuRT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.1 kDa (260aa) confirmed by MALDI-TOF

Additionnal Information

PLUNC, BPI fold-containing family A member 1, BPI fold-containing family A, member 1, bA49G10.5, LPLuNC3, LuNX, NASG, PLuNC, SPuRT, ATGP1908-10 µg, ATGP1908-20 µg, ATGP1908-50 µg, ATGP1908-100 µg, ATGP1908-250 µg, ATGP1908-500 µg, ATGP1908-1 mg, ATGP1908-10, ATGP1908-20, ATGP1908-50, ATGP1908-100, ATGP1908-250, ATGP1908-500, ATGP1908-1

References & Citations

Ghafouri B., et al. (2004) Biochem Biophys Acta. 1699:57-63.; ; Bingle C D., et al. (2002) Hum Mol Genet. 11: 937-943.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_057667

Uniprot Accession Number

Q9NP55

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSQFGGLPV PLDQTLPLNV NPALPLSPTG LAGSLTNALS NGLLSGGLLG ILENLPLLDI LKPGGGTSGG LLGGLLGKVT SVIPGLNNII DIKVTDPQLL ELGLVQSPDG HRLYVTIPLG IKLQVNTPLV GASLLRLAVK LDITAEILAV RDKQERIHLV LGDCTHSPGS LQISLLDGLG PLPIQGLLDS LTGILNKVLP ELVQGNVCPL VNEVLRGLDI TLVHDIVNML IHGLQFVIKV