Recombinant human PYCRL/PYCR3 protein
PYCRL belongs to the pyrroline-5-carboxylate reductase family and functions as a homodecamer. It may play a critical role in proline bio-synthesis. Proline functions as a non-enzymatic antioxidant to minimize damage caused by reactive oxygen species (ROS) in microorganisms, animals and plants. In the last step of proline biosynthesis, PYCRL catalyzes the reduction of aldehyde dehydrogenase 4A1 (ALDH4A1) to proline using NAD (P) H as the cofactor. Recombinant human PYCRL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
31 kDa (297aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nocek B., et al. (2005) J Mol Biol. 354:91-106.; ; Krishnan N., et al. (2008) Free Radic Biol Med. 44: 671-681.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Scientific Category
Metabolism
NCBI Accession Number
AAH07993.1
Uniprot Accession Number
Q53H96
Species
Human
AA Sequence
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