Recombinant human GALT protein
GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Specifications
Product Name Alternative
Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, uDP-glucose--hexose-1-phosphate uridylyltransferase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
45.9 kDa (403aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Reichardt JK. (1992) Hum Mutat. (3) :190-6.; ; Tyfield L, Reichardt J, et al. (1999) . Hum Mutat. (6) :417-30.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_000146.2
Uniprot Accession Number
P07902
Species
Human
AA Sequence
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