Recombinant human GALT protein

GALT catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of uDP-glucose + galactose-1-phosphate to glucose-1-phosphate + uDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human GALT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, uDP-glucose--hexose-1-phosphate uridylyltransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

45.9 kDa (403aa) confirmed by MALDI-TOF

Additionnal Information

GALT, Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, uDP-glucose--hexose-1-phosphate uridylyltransferase, ATGP1904-10 µg, ATGP1904-20 µg, ATGP1904-50 µg, ATGP1904-100 µg, ATGP1904-250 µg, ATGP1904-500 µg, ATGP1904-1 mg, ATGP1904-10, ATGP1904-20, ATGP1904-50, ATGP1904-100, ATGP1904-250, ATGP1904-500, ATGP1904-1

References & Citations

Reichardt JK. (1992) Hum Mutat. (3) :190-6.; ; Tyfield L, Reichardt J, et al. (1999) . Hum Mutat. (6) :417-30.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000146.2

Uniprot Accession Number

P07902

Species

Human

AA Sequence

MSRSGT DPQQRQQASE ADAAAATFRA NDHQHIRYNP LQDEWVLVSA HRMKRPWQGQ VEPQLLKTVP RHDPLNPLCP GAIRANGEVN PQYDSTFLFD NDFPALQPDA PSPGPSDHPL FQAKSARGVC KVMCFHPWSD VTLPLMSVPE IRAVVDAWAS VTEELGAQYP WVQIFENKGA MMGCSNPHPH CQVWASSFLP DIAQREERSQ QAYKSQHGEP LLMEYSRQEL LRKERLVLTS EHWLVLVPFW ATWPYQTLLL PRRHVRRLPE LTPAERDDLA SIMKKLLTKY DNLFETSFPY SMGWHGAPTG SEAGANWNHW QLHAHYYPPL LRSATVRKFM VGYEMLAQAQ RDLTPEQAAE RLRALPEVHY HLGQKDRETA TIA