Recombinant human RNA polymerase III subunit RPC6/POLR3F protein

POLR3F, also known as RPC39 and RPC6, belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family. POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . It is DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR3F protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

DNA-directed RNA polymerase III subunit RPC6, RPC39, RPC6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.1 kDa (339aa) confirmed by MALDI-TOF

Additionnal Information

POLR3F, DNA-directed RNA polymerase III subunit RPC6, RPC39, RPC6, ATGP1897-10 µg, ATGP1897-20 µg, ATGP1897-50 µg, ATGP1897-100 µg, ATGP1897-250 µg, ATGP1897-500 µg, ATGP1897-1 mg, ATGP1897-10, ATGP1897-20, ATGP1897-50, ATGP1897-100, ATGP1897-250, ATGP1897-500, ATGP1897-1

References & Citations

Chiu Y.-H., et al. (2009) Cell 138:576-591; ; Ablasser A., et al. (2009) Nat. Immunol. 10:1065-1072

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006457

Uniprot Accession Number

Q9H1D9

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAEVKVK VQPPDADPVE IENRIIELCH QFPHGITDQV IQNEMPHIEA QQRAVAINRL LSMGQLDLLR SNTGLLYRIK DSQNAGKMKG SDNQEKLVYQ IIEDAGNKGI WSRDIRYKSN LPLTEINKIL KNLESKKLIK AVKSVAASKK KVYMLYNLQP DRSVTGGAWY SDQDFESEFV EVLNQQCFKF LQSKAETARE SKQNPMIQRN SSFASSHEVW KYICELGISK VELSMEDIET ILNTLIYDGK VEMTIIAAKE GTVGSVDGHM KLYRAVNPII PPTGLVRAPC GLCPVFDDCH EGGEISPSNC IYMTEWLEF