Recombinant human TBPL1 protein
TBPL1, also known as STuD, TLF, TLP and TRF2, belongs to the TBP family. The TATA box-binding protein (TBP) is an essential component of the basal transcriptional machinery. Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. It is essential for spermiogenesis and believed to be important in expression of developmentally regulated genes. Recombinant human TBPL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
TATA box-binding protein-like protein 1, MGC8389, MGC9620, STuD, TLF, TLP, TRF2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.3 kDa (209aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ohbayashi T., et al. (1999) Biochem. Biophys. Res. Commun. 255:137-142; ; Rabenstein M.D., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:4791-4796
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT, 1mM EDTA
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004856
Uniprot Accession Number
P62380
Species
Human
AA Sequence
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