Recombinant human AICDA protein

AICDA, also known as AID, is involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. This protein is required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. It may also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Recombinant human AICDA protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Activation-induced cytidine deaminase, AID, ARP2, CDA2, HIGM2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

26.1 kDa (218aa)

Additionnal Information

AICDA, Activation-induced cytidine deaminase, AID, ARP2, CDA2, HIGM2, ATGP1895-10 µg, ATGP1895-20 µg, ATGP1895-50 µg, ATGP1895-100 µg, ATGP1895-250 µg, ATGP1895-500 µg, ATGP1895-1 mg, ATGP1895-10, ATGP1895-20, ATGP1895-50, ATGP1895-100, ATGP1895-250, ATGP1895-500, ATGP1895-1

References & Citations

Guo J.u., et al. (2010) Cell. 145:423-434; ; Noguchi E., et al. (2001) J. Allergy Clin. 108:382-386

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_065712.1

Uniprot Accession Number

Q9GZX7

Species

Human

AA Sequence

MDSLLMNRRK FLYQFKNVRW AKGRRETYLC YVVKRRDSAT SFSLDFGYLR NKNGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT FVENHERTFK AWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL