Recombinant human Fas Ligand/FASLG protein

Fas ligand, also known as FASLG, belongs to the tumor necrosis factor family and is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in FASLG may be related to some cases of systemic lupus erythematosus (SLE) . Recombinant human FASLG protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Fas antigen ligand (Fas ligand; FasL), Tumor necrosis factor ligand superfamily member 6 (TNFSF6), Apoptosis antigen ligand (APTL), CD95 ligand (CD95L), ADAM10-processed FasL form (APL), CD178, APT1LG1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.6 kDa (173aa)

Additionnal Information

FASLG, Tumor necrosis factor ligand superfamily member 6, APT1LG1, CD178, CD95L, FASL, TNFSF6, ATGP1890-10 µg, ATGP1890-20 µg, ATGP1890-50 µg, ATGP1890-100 µg, ATGP1890-250 µg, ATGP1890-500 µg, ATGP1890-1 mg, ATGP1890-10, ATGP1890-20, ATGP1890-50, ATGP1890-100, ATGP1890-250, ATGP1890-500, ATGP1890-1

References & Citations

Kirkin V., et al. (2007) Cell Death Differ. 14:1678-1687; ; Voss M., et al. (2009) BMC Immunol. 10:53-53

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_000630

Uniprot Accession Number

P48023

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MQIGHPSPPP EKKELRKVAH LTGKSNSRSM PLEWEDTYGI VLLSGVKYKK GGLVINETGL YFVYSKVYFR GQSCNNLPLS HKVYMRNSKY PQDLVMMEGK MMSYCTTGQM WARSSYLGAV FNLTSADHLY VNVSELSLVN FEESQTFFGL YKL