Recombinant human Fructosamine-3-kinase/FN3K protein

FN3K catalyzes the phosphorylation of fructosamines which may result in deglycation, the non-enzymatic reaction of glucose with primary amines followed by Amadori re-arrangement. Phosphorylation of fructosamines may initiate metabolism of the modified amine and lead to the de-glycation of fructoselysine and of glycated proteins. Recombinant human FN3K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Fructosamine-3-kinase, Fructosamine 3 kinase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

37 kDa (332aa) confirmed by MALDI-TOF

Additionnal Information

Fructosamine3-kinase, FN3K, Fructosamine-3-kinase, Fructosamine 3 kinase, ATGP1884-10 µg, ATGP1884-20 µg, ATGP1884-50 µg, ATGP1884-100 µg, ATGP1884-250 µg, ATGP1884-500 µg, ATGP1884-1 mg, ATGP1884-10, ATGP1884-20, ATGP1884-50, ATGP1884-100, ATGP1884-250, ATGP1884-500, ATGP1884-1

References & Citations

Conner JR, Beisswenger PJ, et al. (2005) . Ann N Y Acad Sci. 1043:824-36.; ; Delpierre G, Veiga-da-Cunha M, et al. (2006) . Diabetes Metab. 32 (1) :31-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_071441.1

Uniprot Accession Number

Q9H479

Species

Human

AA Sequence

MEQLLRA ELRTATLRAF GGPGAGCISE GRAYDTDAGP VFVKVNRRTQ ARQMFEGEVA SLEALRSTGL VRVPRPMKVI DLPGGGAAFV MEHLKMKSLS SQASKLGEQM ADLHLYNQKL REKLKEEENT VGRRGEGAEP QYVDKFGFHT VTCCGFIPQV NEWQDDWPTF FARHRLQAQL DLIEKDYADR EARELWSRLQ VKIPDLFCGL EIVPALLHGD LWSGNVAEDD VGPIIYDPAS FYGHSEFELA IALMFGGFPR SFFTAYHRKI PKAPGFDQRL LLYQLFNYLN HWNHFGREYR SPSLGTMRRL LK