Recombinant human FDCSP protein
FDCSP is a small secreted protein that is in follicular dendritic cells. This protein specifically binds to activated B cells, and functions as a regulator of antibody responses. It is also thought to contribute to tumor metastases by promoting cancer cell migration and invasion. Recombinant human FDCSP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Follicular dendritic cell secreted peptide, C4orf7, FDC-SP
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
10.4 kDa (91aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wang, C., et al. (2010) Oncol. Rep. 24 (4), 933-939; ; Al-Alwan, M., et al. (2007) . Immunol. 178 (12), 7859-7867;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.15M NaCl, 50% glycerol, 1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_694542.1
Uniprot Accession Number
Q8NFU4
Species
Human
AA Sequence
FPVSQDQ EREKRSISDS DELASGFFVF PYPYPFRPLP PIPFPRFPWF RRNFPIPIPE SAPTTPLPSE K
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