Recombinant human CD3 zeta/CD247 protein
T-cell surface glycoprotein CD3 zeta chain, also known as CD247, belongs to the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Recombinant human CD247 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
TCRZ, T-cell surface glycoprotein CD3 zeta chain isoform 1, T3Z, IMD25, CD3-ZETA, CD3Z, CD3Q, CD3H, CD247
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.4 kDa (136aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46; ; Rieux-Laucat F., et al. (2006) N. Engl. J. Med. 354:1913-1921;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_932170.1
Uniprot Accession Number
P20963
Species
Human
AA Sequence
RVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPR
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
DDIT4L Polyclonal Antibody, PE-Cy5 Conjugated
EITH-L-GB-WASH-4A Scafftag Tagging System Kits