Recombinant human CD3 zeta/CD247 protein
T-cell surface glycoprotein CD3 zeta chain, also known as CD247, belongs to the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Recombinant human CD247 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
TCRZ, T-cell surface glycoprotein CD3 zeta chain isoform 1, T3Z, IMD25, CD3-ZETA, CD3Z, CD3Q, CD3H, CD247
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.4 kDa (136aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46; ; Rieux-Laucat F., et al. (2006) N. Engl. J. Med. 354:1913-1921;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Immunology
NCBI Accession Number
NP_932170.1
Uniprot Accession Number
P20963
Species
Human
AA Sequence
RVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPR
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