Recombinant human BTF3 protein

Basic transcription factor 3, also known as BTF3, forms a stable complex with RNA polymerase II. This protein is required for the initiation of transcription. Recombinant human BTF3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Transcription factor BTF3, BETA-NAC, BTF3a, BTF3b, NACB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

24.6 kDa (229aa) confirmed by MALDI-TOF

Additionnal Information

BTF3, ATGP1870-10 µg, ATGP1870-20 µg, ATGP1870-50 µg, ATGP1870-100 µg, ATGP1870-250 µg, ATGP1870-500 µg, ATGP1870-1 mg, ATGP1870-10, ATGP1870-20, ATGP1870-50, ATGP1870-100, ATGP1870-250, ATGP1870-500, ATGP1870-1

References & Citations

Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17; ; Matsuoka S., et al. (2007) Science. 316:1160-1166;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001032726

Uniprot Accession Number

P20290

Species

Human

AA Sequence

MRRTGAP AQADSRGRGR ARGGCPGGEA TLSQPPPRGG TRGQEPQMKE TIMNQEKLAK LQAQVRIGGK GTARRKKKVV HRTATADDKK LQFSLKKLGV NNISGIEEVN MFTNQGTVIH FNNPKVQASL AANTFTITGH AETKQLTEML PSILNQLGAD SLTSLRRLAE ALPKQSVDGK APLATGEDDD DEVPDLVENF DEASKNEAN