Recombinant human BTF3 protein
Basic transcription factor 3, also known as BTF3, forms a stable complex with RNA polymerase II. This protein is required for the initiation of transcription. Recombinant human BTF3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Transcription factor BTF3, BETA-NAC, BTF3a, BTF3b, NACB
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
24.6 kDa (229aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17; ; Matsuoka S., et al. (2007) Science. 316:1160-1166;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001032726
Uniprot Accession Number
P20290
Species
Human
AA Sequence
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