Recombinant human HFE protein

HFE, also known as HLA-H, belongs to the MHC class I family. This protein contains 1 Ig-like C1-type (immunoglobulin-like) domain. HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta-2 microglobulin (beta2M) . It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor (TFR) with transferrin. It binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin. Recombinant human HFE protein, fused to His-tag at N-terminus, was expressed in E. coli.

Product Specifications

Product Name Alternative

Hereditary hemochromatosis protein, HFE1, HH, HLA-H, MVCD7, TFQTL2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Denatured

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

35.7 kDa (308aa)

Additionnal Information

HFE, Hereditary hemochromatosis protein, HFE1, HH, HLA-H, MVCD7, TFQTL2, ATGP1869-10 µg, ATGP1869-20 µg, ATGP1869-50 µg, ATGP1869-100 µg, ATGP1869-250 µg, ATGP1869-500 µg, ATGP1869-1 mg, ATGP1869-10, ATGP1869-20, ATGP1869-50, ATGP1869-100, ATGP1869-250, ATGP1869-500, ATGP1869-1

References & Citations

Feder J.N., et al. (1998) Proc. Natl. Acad. Sci. u.S.A. 95:1472-1477; ; West, A P, et al. (2000) J. Biol. Chem. (uNITED STATES) 275 (49) : 38135-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000401.1

Uniprot Accession Number

Q30201

Species

Human

AA Sequence

RLLRSH SLHYLFMGAS EQDLGLSLFE ALGYVDDQLF VFYDHESRRV EPRTPWVSSR ISSQMWLQLS QSLKGWDHMF TVDFWTIMEN HNHSKESHTL QVILGCEMQE DNSTEGYWKY GYDGQDHLEF CPDTLDWRAA EPRAWPTKLE WERHKIRARQ NRAYLERDCP AQLQQLLELG RGVLDQQVPP LVKVTHHVTS SVTTLRCRAL NYYPQNITMK WLKDKQPMDA KEFEPKDVLP NGDGTYQGWI TLAVPPGEEQ RYTCQVEHPG LDQPLIVIWE PSPSGTLV