Recombinant human COX5A protein
COX5A, also known as COX, COX-VA and VA, belongs to the cytochrome c oxidase subunit 5A family. Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. COX5A is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Recombinant human COX5A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
14.9 kDa (132aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lenka N, et al. (1998) . Prog. Nucleic Acid Res. Mol. Biol. 61: 309-44.; ; Schmidt, T.R., et al. (2002) Gene 286: 13-19.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 2mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_004246
Uniprot Accession Number
P20674
Species
Human
AA Sequence
SHGSQET DEEFDARWVT YFNKPDIDAW ELRKGINTLV TYDMVPEPKI IDAALRACRR LNDFASTVRI LEVVKDKAGP HKEIYPYVIQ ELRPTLNELG ISTPEELGLD KV
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
