Recombinant human LSM12 protein

LSM12 belongs to the snRNP Sm proteins family. Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. Recombinant human LSM12 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein LSM12 homolog, Protein LSM12 homolog, PNAS-135

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.1 kDa (218aa) confirmed by MALDI-TOF

Additionnal Information

LSM12, Protein LSM12 homolog, Protein LSM12 homolog, PNAS-135, ATGP1865-10 µg, ATGP1865-20 µg, ATGP1865-50 µg, ATGP1865-100 µg, ATGP1865-250 µg, ATGP1865-500 µg, ATGP1865-1 mg, ATGP1865-10, ATGP1865-20, ATGP1865-50, ATGP1865-100, ATGP1865-250, ATGP1865-500, ATGP1865-1

References & Citations

Lehner, Ben, et al. (2004) Genome Res. (united States) 14 (7) : 1315-23.; ; Achsel T., et al. (1999) EMBO J. 18:5789-5802;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_689557

Uniprot Accession Number

Q3MHD2

Species

Human

AA Sequence

MAAPPGE YFSVGSQVSC RTCQEQRLQG EVVAFDYQSK MLALKCPSSS GKPNHADILL INLQYVSEVE IINDRTETPP PLASLNVSKL ASKARTEKEE KLSQAYAISA GVSLEGQQLF QTIHKTIKDC KWQEKNIVVM EEVVITPPYQ VENCKGKEGS ALSHVRKIVE KHFRDVESQK ILQRSQAQQP QKEAALSS