Recombinant human VBP1 protein
Prefoldin subunit 3, also known as VBP1, belongs to the prefoldin subunit alpha family. VBP1 interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. Recombinant human VBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Prefoldin subunit 3, PFD3, PFDN3, VBP-1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25 kDa (220aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kim S Y., et al. (2008) BMB Rep. 41 (2) :158-63.; ; Mousnier A., et al. (2007) Proc Natl Acad Sci uSA. 201 (34) :13612-20.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_003363
Uniprot Accession Number
P61758
Species
Human
AA Sequence
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